![]() ![]() These enzymes offer improved elongation rates, reduced error rates, and increased efficiency for NGS applications. These include such enzymes as Phusion® DNA polymerase, Q5® DNA polymerase from NEB, and many others. The enzyme is commonly used for cloning and sequencing applications, however it is relatively slow, sensitive to contaminants and inhibitors and has low processivity resulting in usually low PCR yields.Īlternative, mostly better DNA polymerases have been developed by different companies to address the limitations of Pfu polymerase. Pfu polymerase has 3' to 5' exonuclease proofreading activity, which enables it to correct errors that occur during DNA synthesis. It is a thermostable Pfu polymerase that was widely used in molecular biology applications for its high fidelity of DNA amplification due to a high proofreading activity. ![]() Pfu DNA Polymerase was originally found in thermophilic bacterium Pyrococcus furiosus. ![]()
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